Join Blue Frog Scientific at SETAC EU 2026: Advancing Bioaccumulation Assessment with NAMs

Blue Frog Scientific will be presenting new research at SETAC Europe 2026, exploring how New Approach Methodologies (NAMs) can transform the way we assess bioaccumulation – particularly for complex and difficult substances.

Presentation ID: 1.03.P-Tu022
Presenter: Pauline Remuzat
Visit us at Booth 79

Rethinking Bioaccumulation Assessment

Traditional bioaccumulation screening relies heavily on log K_ow as a surrogate metric. While widely used, this approach has limitations – particularly for substances that do not follow simple lipid partitioning behaviour.

As highlighted in the poster, log K_ow-based assessments can fall short for surfactants, ionisable compounds, and PFAS, where alternative mechanisms such as protein binding, membrane interactions, and biotransformation play a critical role.

A New Strategy for Complex Substances

Our work introduces a non-animal testing strategy combining:

• In chemico and in vitro assays.
• In silico modelling approaches.
• Weight-of-evidence (WoE) frameworks.

This integrated approach evaluates multiple parameters including:

• Lipid partitioning (K_ow).
• Membrane interactions (K_mw).
• Protein binding (K_bsa).
• Respiratory uptake (K_oa).
• Biotransformation and depuration.

Together, these provide a more accurate and mechanistically relevant understanding of bioaccumulation behaviour .

Improving Predictability and Reducing Animal Testing

By combining experimental data with tools such as the Bioaccumulation Assessment Tool (BAT) and other QSAR models, this strategy enhances:

• Precision and predictability of BCF estimates.
• IVIVE (in vitro–in vivo extrapolation).
• Confidence in regulatory decision-making.

Importantly, this approach supports PBT assessment frameworks while reducing reliance on animal testing, aligning with evolving regulatory expectations.

Why This Matters for Industry

As regulatory scrutiny increases – particularly for PFAS and other challenging substance classes – there is a growing need for:

• More robust, science-led assessment strategies.
• Greater mechanistic understanding of bioaccumulation.
• Approaches that go beyond traditional screening tools.

This work represents a step toward future-ready bioaccumulation assessment, combining innovation with regulatory relevance.

Meet the Blue Frog Team at SETAC EU 2026

We’d love to hear your views and discuss how these approaches could support your regulatory challenges.

Visit us at Booth 79, attend Pauline's presentation and speak with our team about applying NAMs to your substances.

Download a copy of Pauline's poster here...